Avoid large changes in volume during boiling; put a loose lid on the container to protect from evaporation. For 1 L: 24 g Tris base formula weight: For a 1x solution, mix 1 part of the 10x solution with 9 parts distilled water and adjust pH to 7.
This avoids the large volume of potentially hazardous hydrochloric acid that is needed to neutralize a solution of Tris base alone. For 1 L: 24 g Tris-HCl formula weight: For mL: 6.
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